Benchmark Scientific - E4500-LD
SmartGlow Loading Dye with Safe Green Stain, 1.0ml
Product Description:
Benchmark Accuris SmartGlow DNA Stains are ideal for excitation of nucleic acids with UV or visible blue light transilluminators. Unlike EtBR, Benchmark Accuris SmartGlow DNA Stains are non-mutagenic and do not require special methods of disposal.
Protocol for SmartGlow PS
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Prepare 100 mL of agarose or polyacrylamide solution.
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Add 5 µl of SmartGlow PS stain to the gel solution before pouring gels.
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For enhanced results, add SmartGlow PS to the running buffer at a ratio of 2.5 µl to 5 µl per 100 mL. Adding SmartGlow PS to the running buffer will result in increased sensitivity & better detection of small quantities of nucleic acid.
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After electrophoresis is complete, view the gel using a UV or blue light illuminator
Protocol for SmartGlow LD
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Prepare agarose for polyacrylamide solution.
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Pour gels.
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Mix SmartGlow LD with samples & DNA markers at a 1:5 (dye to sample) dilution.
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After electrophoresis is complete, view the gel using a UV or blue light illuminator.