FroggaBIO USA Inc - PVR004

The Plant Virus RNA Kit uses a simple and efficient spin column procedure to purify virus RNA from a wide range of virus infected plant species including those with high levels of polysaccharide inhibitors

In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the spin column

The procedure does not require phenol extraction or alcohol precipitation and can be completed within 25 minutes A variety of high-qualityplant viral RNA can be purified from 100 mg plant tissue samples and can be used directly in a variety of sensitive downstream applications

Applications

• RT-PCR
• RT-qPCR
• Next Generation Sequencing (NGS)
• Northern Blotting

• Components

  • PVR Buffer
  • PVRS Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • PV Columns
  • 2 ml Collection Tubes

  • Quality Control

    The Plant Virus RNA Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system Virus RNA is purified from leaf discs of grapevine plants infected with multiple viruses Following RNA purification a 5 µl aliquot from a 50 µl eluate of purified viral RNA is analyzed by electrophoresis on a 08% agarose gel