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Eppendorf - innova44 44r incubated shaker

The Innova 44 incubator shaker and 44R with added refrigeration are stackable shakers that provide very broad temperature capabilities for culturing a wide variety of organisms in flasks up to 5 L.

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M1282-0010

M1282-0010| Eppendorf New Brunswick™ Innova® 44, 120 V, orbit diameter 5.1cm (2in)

$21,107.90 $20,255.06
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M1282-0010

M1282-0010| Eppendorf New Brunswick™ Innova® 44, 120 V, orbit diameter 5.1cm (2in)

$21,107.90 $20,255.06
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M1282-0010

M1282-0010| Eppendorf New Brunswick™ Innova® 44, 120 V, orbit diameter 5.1cm (2in)

$21,107.90 $20,255.06
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M1282-0004

Eppendorf New Brunswick Innova 44R Incubated Refrigerated Shaker, Orbit Diameter of 2.5cm (1in). 120 V/60 Hz, US Plug

$24,048.20 $23,076.56
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M1282-0004

Eppendorf New Brunswick Innova 44R Incubated Refrigerated Shaker, Orbit Diameter of 2.5cm (1in). 120 V/60 Hz, US Plug

$24,048.20 $23,076.56
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Product Description:

The Innova 44 incubator shaker and 44R with added refrigeration are stackable shakers that provide very broad temperature capabilities for culturing a wide variety of organisms in flasks up to 5 L.

user_manual

Technical Specifications

Innova® 44, orbit diameter 2.5 cm (1 in) Innova® 44R, orbit diameter 2.5 cm (1 in) Innova® 44, orbit diameter 5.1 cm (2 in) Innova® 44R, orbit diameter 5.1 cm (2 in)
External height (with lid/door open) 119.3 cm (47 in) 119.3 cm (47 in) 119.3 cm (47 in) 119.3 cm (47 in)
Timer 0.01 – 99.59 h, continuous 0.01 – 99.59 h, continuous 0.01 – 99.59 h, continuous 0.01 – 99.59 h, continuous
Power supply 120 V, 60 Hz 120 V, 60 Hz 120 V, 60 Hz 120 V, 60 Hz
Dimensions (W?×?D?×?H) 135?× 87.3?× 66 cm / 53.1?× 33.2?× 78 in 135?× 87.3?× 66 cm / 53.1?× 33.2?× 78 in 135?× 87.3?× 66 cm / 53.1?× 33.2?× 78 in 135?× 87.3?× 66 cm / 53.1?× 33.2?× 78 in
Weight w/o accessories 238kg / 523 lb 257kg / 566 lb 238kg / 523 lb 257kg / 566 lb
Temperature range Ambient +5 °C to 80 °C 20 °C below ambient to 80 °C Ambient +5 °C to 80 °C 20 °C below ambient to 80 °C
Audible and visual alarms yes yes yes yes
Temperature uniformity ±0.25 °C at 37 °C ±0.25 °C at 37 °C ±0.25 °C at 37 °C ±0.25 °C at 37 °C
Available program modes › Constant speed and temperature › Constant speed and temperature › Constant speed and temperature › Constant speed and temperature
› Programmable multi-steps › Programmable multi-steps › Programmable multi-steps › Programmable multi-steps
› RS-232 communication port › RS-232 communication port › RS-232 communication port › RS-232 communication port
Balanced drive mechanism Triple-eccentric with permanently lubricated ball bearings2 Triple-eccentric with permanently lubricated ball bearings2 Triple-eccentric with permanently lubricated ball bearings2 Triple-eccentric with permanently lubricated ball bearings2
Benchtop (B), Under Bench (U), Floor (F) or Stackable (S) F, S (x3) F, S (x3) F, S (x3) F, S (x3)
Gassing manifold yes yes yes yes
Humidity monitor yes yes yes yes
Internal convenience light yes yes yes yes
Memory Non volatile with automatic power failure restart Non volatile with automatic power failure restart Non volatile with automatic power failure restart Non volatile with automatic power failure restart
Motor type Solid state, DC brushless motor Solid state, DC brushless motor Solid state, DC brushless motor Solid state, DC brushless motor
Orbit 2.5 cm (1 in) 2.5 cm (1 in) 5.1 cm (2 in) 5.1 cm (2 in)
Photosynthetic lighting no yes no yes
Platform size 76 × 46 cm (30 × 18 in) 76 × 46 cm (30 × 18 in) 76 × 46 cm (30 × 18 in) 76 × 46 cm (30 × 18 in)
Refrigerated yes yes
Slide out platform yes yes yes yes
Space saving glide up doors yes yes yes yes
Speed range1 25 – 400 rpm with 2.5 cm orbit; 25 – 300 rpm with 5.1 cm orbit or 2 stacked units; 25 – 250 rpm with 3 stacked units 25 – 400 rpm with 2.5 cm orbit; 25 – 300 rpm with 5.1 cm orbit or 2 stacked units; 25 – 250 rpm with 3 stacked units 25 – 400 rpm with 2.5 cm orbit; 25 – 300 rpm with 5.1 cm orbit or 2 stacked units; 25 – 250 rpm with 3 stacked units 25 – 400 rpm with 2.5 cm orbit; 25 – 300 rpm with 5.1 cm orbit or 2 stacked units; 25 – 250 rpm with 3 stacked units
Multi-step programming yes yes yes yes
Steps per program 15 15 15 15
Stored programs 4 4 4 4
UV germicidal lamp no yes no yes
1Baffled flasks will reduce maximum speed.

Frequently Asked Questions

How does the shaker agitation rate and orbit affect culture growth?

  • Generally, agitation speed and orbit affect both the aeration and the mixing of the culture. Greater aeration increases oxygen transfer rate (OTR), boosting cell growth. The size of the orbit affects OTR linearly and the speed has a square-law relationship. Therefore, if the size of the orbit doubles, say from 1/2 to 1," the amount of OTR should double?RPM?_1/?RPM?_2 = (r_2^2)/(r_1^2 ), then ?RPM?_2 = √(?RPM?_1^2 (r_1/r_2 ) ). If the speed doubles, the OTR should quadruple. Of course, these are general rules and caution should be taken with shear sensitive cells. However, this information can be used to estimate the conditions needed to obtain the same results on different model shakers. For example, to get the same oxygen transfer in a shaker with a 1 in orbit running at 300 rpm, a shaker with 3/4 in orbit must be run at 350 rpm."

Do New Brunswick Scientific shakers come with platforms?

  • Each New Brunswick shaker is available with multiple platform options, which are ordered separately to give you the flexibility to choose the one that best suits your needs. Dedicated platforms include installed clamps of a single size (ie. 250 mL clamps) and provide the greatest capacity but does not allow for the use of different size clamps or holders. The most popular platforms are Universal Platforms which are designed for use with a combination of various-sized clamps and test tube racks providing more flexibility. When ordering a Universal Platform, clamps and test tube racks must also be ordered separately. For applications running below 250 rpm Sticky Tape and/or Sticky Pad are also available in lieu of clamps. (However Sticky Pad and Sticky Tape cannot be used in waterbath shakers)

What is the temperature offset function?

  • The temperature offset function on many of our shakers allow you to match a reference point" temperature inside the chamber (culture medium) with the "Actual Value" displayed. The temperature offset function on Innova Incubated shakers allows you to monitor the temperature of the media providing you a clearer and more accurate picture. Please be aware that matching reference point temperatures to the Actual means you have to deal with many variables such as: greater evaporation; heat gains generated by cultures growing - (slowly at first and much more during their growth phase) and heat generated by the friction of media with the glass at high speeds or use of baffled flasks. Over time, the reference point temperature offset may need to change due to these varying conditions.

I want to grow my cultures at 27°C, do I need an incubated shaker with refrigeration?

  • Our New Brunswick Incubated, non-refrigerated Shakers Innova and I-Series are designed to maintain temperatures of ambient plus 5 °C to its maximum operating temperature. The New Brunswick Incubated Excella shakers will operate from ambient plus 7 °C to maximum temperature. It is critical to understand that "ambient temperature is not the temperature on the wall thermometer (room temperature), but the temperature measured one meter from the front of the machine."

Why are some flask clamps supplied with an extra spring set?

  • This "extra spring set" (we call it a girdle) is included with clamps for 2 L, 2.8 L, 4 L and 6 L flasks. It is intended to be used with the already installed spring set. This girdle helps prevent large flasks from spinning inside the clamp and reduces the risk of flasks becoming dislodged or scored by the steel clamp. One girdle is already in place on the clamp, the other is packed separately. To install this extra “girdle”, follow the instructions in the manual supplied with the shaker."

How is temperature accuracy defined by Eppendorf?

  • Accuracy is defined as the degree of closeness between the displayed value and the actual value as determined by a traceable device. All New Brunswick Incubated Innova and I-Series shakers have a temperature Accuracy of ±0.1 °C from 30 – 40 °C, ±0.5 °C over the remaining range. New Brunswick Incubated Excella Shakers are accurate within 0.1°C at 37°C.

How is Uniformity defined by Eppendorf?

  • Uniformity is the ability to maintain uniform temperatures throughout the platform. New Brunswick Incubated Innova Shaker temperatures will not deviate by more than +/- 0.25 °C at 37 °C.

What is an orbit?

  • The orbit of a shaker is the distance traveled around the central drive. This can be described in inches, centimeters, or millimeters. If our shakers had a one inch (2.54 cm orbit. That means that that the lateral movement of the platform would equal one inch from one point to another that is 180° opposite that position. All of the New Brunswick. shakers have a circular orbit

Which rpms and orbits are recommended for my culture (bacteria, fungi, yeast, cells, algae..)?

  • Recommended orbits for culturing: Bacteria: 250 rpm, Fungi: 200 rpm, Yeast: 120 - 300 rpm, Insect cells: 100 rpm and Algae: 110 rpm. These are general guidelines that should be optimized for your process.

What wavelengths are used in your growth lamps in a photosynthetic light bank?

  • Each of the bulbs that is fit into our photobanks is a wide spectrum bulb that provides the red and blue wavelength of energy needed for photosynthesis. Innova 42R has six bulbs as the Innova 43R and 44R have nine bulbs. To have this option installed, the shaker needs to have the refrigeration option installed as well.

Which screws will fit to which platform?

  • All of Eppendorf Inc. clamps and accessories are packaged with multiple screws to fix them to our universal platforms, included in M1013-0261 clamp screw kit (Stainless steel platform screw #S2117-3050 (10-32 x 5/16 in (7.9mm)), Stainless Steel, Aluminum, and Aluminum and Phenolic (Plastic) #S2116-3051 (10-24 x 5/16 in (7.9 mm)), and Wood #S2116-3101 (10-24 x 5/8 in (15.87mm)).

How can I store and monitor my data?

  • BioCommand SFI is the tracking program that can record/monitor the shaker speed, temperature and humidity. The program software is downloaded into your PC and registered with Eppendorf Inc. This will store information until the PC's hard drive is full.

How can I disinfect, clean, maintain my shaker?

  • We recommend cleaning the exterior of our units with a damp cloth or any standard household or laboratory cleaner to wipe the surfaces clean. Do not use abrasive or corrosive compounds to clean the instruments as they may damage the unit and void the warranty. It is the responsibility of the user to carry out the proper decontamination procedures if hazardous materials are spilled on or inside the equipment. Before using any cleaning or decontamination method other than those suggested by the manufacturer, users should contact Eppendorf Inc. to ensure that the proposed method will not damage the equipment. Commercially available household bleach solutions, when diluted at a 1:10 ratio, are effective in routine decontamination of the instrument. The method for decontaminating a spill depends on the nature of the spill. Spills involving fresh cultures or samples of known concentrations of biomass should be flooded with decontamination solution and soaked for at least 5 minutes before cleanup. Spills involving samples with high concentrations of biomass, or involving organic matter, or occurring in areas warmer than ambient/room temperature, should be exposed to decontamination solution for at least one hour before being cleaned up. Personnel involved in the cleanup of any spill should wear gloves, safety glasses, and a laboratory coat or gown during the cleanup process. Respiratory protection should be considered for spills where aerosolization is suspected.

What is the difference between a drip pan and a spill cover?

  • The spill cover is 46 x 39 cm (18 x 15.37 in) and comes with the unit. The drip pan is 46 x 46 cm (18 x 18 in) and is ordered as an accessory for any 46 cm (18 x 18 in) platform. The incubated line of shakers comes with the spill cover, whereas the open-air shakers do not. The drip pan can be added below a universal platform, but it cannot be used with the spill cover.

Which flask type fits in which clamp?

  • Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the User guides section of the shaker product page.

Which vessel fits in Eppendorf Test tube racks?

  • Please see the Accessories for New Brunswick™ Shakers: Dimensions and Capacities in the User guides section of the shaker product page.

What is the humidity option? Is the value of humidity shown in the display? How can I avoid evaporation?

  • The humidity monitoring option that is offered with our Innova line displays the Relative Humidity (RH) of the chamber. This value will be reported if the shaker is equipped with the humidity sensor. The value can be shown in the main display screen if programmed to do so or the value can be viewed with all other parameters under the SUMM (summary) screen. The calibrated sensor is accurate within 3 % up to 95 % RH and 5 % up to 99 % RH. To avoid evaporation in the shakers, it is recommended to fill the humidification reservoir beneath the platform, this will mitigate loss of culture volume.

Why is the triple eccentric drive special? What is the difference between Innova and Excella Shakers drive?

  • The Innova and I-series Triple eccentric drive consists of three individual bearings and eccentric shafts mounted in a triangular fashion for precise uniform circular motion at every point on the platform. Recommended for heavier loads. The heavy duty drive has a single shaft that utilizes springs to aid in balancing the load. This drive works great with small to medium loads at lower speeds.

How can I shake MTPs? Which parameters?

  • New Brunswick Shakers manufactured by Eppendorf offer a variety of Micro Titer Plate holders that attach to our universal platforms. Our shakers offer a variety of orbits up to 5.1 cm (2 in) and speeds up to 500 rpm. If mixing is what is needed at higher speeds and smaller orbits you can find Eppendorf Mixers.

What is the heavy load platform?

  • The Heavy Duty Platform (M1282-9928) is designed for use in the Innova 44 only. This platform is 0.95 cm thick aluminum plate that is recommended for shaking full loads of flasks greater than 1 L at or above 20 % fill volumes or flasks with baffles.

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