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Eppendorf Mastercycler Nexus Thermocycler

Mastercycler Nexus with it’s four different block versions is your reliable companion when it comes to the daily routine of PCR

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Eppendorf Mastercycler Nexus Thermocycler Product Description

Mastercycler Nexus with it’s four different block versions is your reliable companion when it comes to the daily routine of PCR

Technical Specifications

MC nexus flat eco MC nexus gradient MC nexus eco MC nexus MC nexus gradient eco MC nexus flat MC nexus GSX1 MC nexus SX1 MC nexus GSX1e MC nexus SX1e
Thermoblock Aluminum Aluminum Aluminum Aluminum Aluminum Aluminum Silver Silver Silver Silver
Temperature control range of the block 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C 4 – 99 °C
Temperature control mode Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe Fast, Standard, Safe
Heating technology of the block Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology Peltier elements, Triple Circuit Technology
Intuitive graphic programming
E-mail Notification
Sample capacity 4 slides or other matching consumables 96 × 0.2 mL PCR tubes, 1 PCR plate 8 × 12 or 38 × 0.5 mL PCR tubes 96 × 0.2 mL PCR tubes, 1 PCR plate 8 × 12 or 38 × 0.5 mL PCR tubes 96 × 0.2 mL PCR tubes, 1 PCR plate 8 × 12 or 38 × 0.5 mL PCR tubes 96 × 0.2 mL PCR tubes, 1 PCR plate 8 × 12 or 38 × 0.5 mL PCR tubes 4 slides or other matching consumables 96 × 0.2 mL PCR vessels, 1 PCR plate 8 × 12 96 × 0.2 mL PCR vessels, 1 PCR plate 8 × 12 96 × 0.2 mL PCR vessels, 1 PCR plate 8 × 12 96 × 0.2 mL PCR vessels, 1 PCR plate 8 × 12
flexlid™ concept
2 years warranty
Silver block
Gradient range 1 – 20 °C 1 – 20 °C 1 – 20 °C 1 – 20 °C
Gradient block
Gradient temperature range 30 – 99 °C 30 – 99 °C 30 – 99 °C 30 – 99 °C
Lid temperature range 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C 37 – 110 °C
Unit can control 2 other units in network
Unit can only be controlled1
Lid descent and closing pressure flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP flexlid®technology, with TSP
USB port
Block homogeneity 20–72 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C ≤ ±0.3 °C
Block homogeneity 90 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C ≤ ±0.4 °C
Block temperature accuracy ±0.2 °C ±0.2 °C ±0.2 °C ±0.2 °C ±0.2 °C ±0.2 °C ±0.2 °C ±0.2 °C ±0.2 °C ±0.2 °C
Heating rate 3 °C/s 2 3 °C/s 2 3 °C/s 2 3 °C/s 2 3 °C/s 2 3 °C/s 2 5 °C/s 2 5 °C/s 2 5 °C/s 2 5 °C/s 2
Cooling rate 2 °C/s 2 2 °C/s 2 2 °C/s 2 2 °C/s 2 2 °C/s 2 2 °C/s 2 3.5 °C/s 2 3.5 °C/s 2 3.5 °C/s 2 3.5 °C/s 2
Gradient block Over 12 rows (0.2 mL) Over 12 rows (0.2 mL) Over 12 rows (0.2 mL) Over 12 rows (0.2 mL)
Interfaces CAN_in/CAN_out, Ethernet CAN_in/CAN_out, Ethernet, USB CAN_in/CAN_out, Ethernet CAN_in/CAN_out, Ethernet, USB CAN_in/CAN_out, Ethernet CAN_in/CAN_out, Ethernet, USB CAN_in/CAN_out, Ethernet, USB CAN_in/CAN_out, Ethernet, USB CAN_in/CAN_out, Ethernet CAN_in/CAN_out, Ethernet
Total weight 10.4 kg 10.9 kg 10.9 kg 10.9 kg 10.4 kg 10.4 kg 10.9 kg 10.9 kg 10.4 kg 10.4 kg
Power supply 110 V, 50 – 60 Hz 115 V, 50 – 60 Hz 115 V, 50 – 60 Hz 115 V, 50 – 60 Hz 110 V, 50 – 60 Hz 110 V, 50 – 60 Hz 110 V, 50 – 60 Hz 110 V, 50 – 60 Hz 110 V, 50 – 60 Hz 110 V, 50 – 60 Hz
Max. power consumption 700 W 700 W 700 W 700 W 700 W 700 W 540 W 540 W 540 W 540 W
Dimensions (W × D × H) 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm 25 × 41.2 × 32.1 cm
Number of programs on device > 700 > 700 > 700 > 700 > 700 > 700 > 700 > 700 > 700 > 700
1 Control through a Mastercycler® nexus, Mastercycler® nexus gradient, Mastercycler® nexus X1g, Mastercycler® nexus X1 or Mastercycler® nexus flat
2 Measured at the block

Eppendorf White Papers and Application Notes

Eppendorf White Paper 032- Eppendorf Mastercycler®: Meeting all Your PCR Needs with Reliability and Flexibility

Straightforward PCR Optimization and Highly Flexible Operation on the Dual Block Thermocycler Mastercycler® Nexus GX2- Application Note 289

Comparative Run Time Evaluations of PCR Thermal Cyclers- Application Note 274

Imaging of Single Cell Gene Expression via In Situ RT-PCR on the Eppendorf Mastercycler® Nexus Flat- Applicatoin Note 249

Excellent Temperature Homogeneity Contributes to High PCR Performance of the Mastercycler® Nexus- Application Note 244

The Mastercycler® Nexus Combines High Energy Efficiency with Very Low Noise Generation- Application Note 243

Poster - Getting the Most Out of Your PCR

User Manuals

Mastercycler Nexus Operating Manual

Mastercycler Nexus Pro Self-Test Dongle Instructions for Use

It is easy to use, does not need much space or energy & sends you an e-mail when it is done.

Combine, Connect, Control

  • Combine up to 3 units for maximum throughput
  • Connect your Mastercycler nexus to your computer network & get a status e-mail to your desk!
  • Control all the relevant parameters of your PCR through the intuitive software

Mastercycler nexus

  • Universal block for plates, 0.1 mL, 0.2 mL & 0.5 mL PCR tubes

Mastercycler nexus X1

  • 96 well silver block for increased speed with 0.1 mL & 0.2 mL tubes & plates

Mastercycler nexus flat

  • Special consumables like slides can be heated & cooled without an adapter

Popular Applications

  • Standard PCR
  • Cycle sequencing
  • In situ PCR

Eppendorf Thermocycler Brochure

Eppendorf PCR Consumables Brochure

Features & Benefits

  • Flexlid Concept
  • Due to the flexlid concept, the cycler can deal with all types of PCR consumables, from low volume all the way up to 0.5 mL PCR tubes
  • SteadySlope® Technology
  • Ensures that the heating & cooling ramp rates are identical in both gradient & normal operation
  • Extreme consumables flexibility
  • Small footprint
  • Intuitive graphic programming (optional mouse use)
  • Optional gradient for PCR optimization
  • Up to two other units can be connected to a central unit
  • E-mail notification
  • Flexlid® concept: automatic height adjustment of the lid allows you to use all types of consumables
  • Optional self-test functionality
  • 2-year warranty

Certified Quality

All Eppendorf cyclers follow these quality guidelines:

  • Individual, documented quality control certificates
  • Calibration accordingly to national & international standards: NIST® (USA), DKD / PTB (Germany), UKAS / NPL (UK) >uL / cUL listed
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