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FroggaBio USA Inc - Geneaid Midi Plasmid Kit

The Geneaidandtrade;andnbsp;Midi Plasmid Kitandnbsp;uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells

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PI002

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FroggaBio USA Inc - Geneaid Midi Plasmid Kit Product Description

The Geneaidandtrade;andnbsp;Midi Plasmid Kitandnbsp;uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells

The Geneaid™ Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells

TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization

  • A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNARNA contaminants
  • Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed
  • The purified plasmid DNA is eluted then precipitated with isopropanol for desalting
  • The entire procedure can be completed without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun
  • **Available Endotoxin-Free, see product details below!** 
    • Applications

      • Transfection
      • Sequencing
      • Ligation
      • PCR
      • In-vitro Transcription
      • Microinjection
      • Restriction Enzyme Digestion
      • Gene Gun
      • Components
          • PM1, PM2, PM3 Buffer
          • TrueBlue Lysis Buffer
          • PEQ Buffer
          • PW Buffer
          • PEL Buffer
          • RNase A
          • Plasmid Midi Columns

Specifications:

Format: Anion-Exchange Gravity Flow

Binding Capacity: 800 µg

Presto™ Column: NA

Endotoxin Level: <015 EUµg

Culture Input: 50-300 ml

Plasmid Size: 1-20 kb

Typical Yield: 200-800 µg

Elution Volume: 500 µl-2 ml

Operation Time: <120 minutes


SpecificationsPresto™ Midi Plasmid KitPresto™ Midi Plasmid Kit Endotoxin FreeGeneaid™ Midi Plasmid KitGeneaid™ Midi Plasmid Kit Endotoxin FreeGeneaid™ Maxi Plasmid KitGeneaid™ Maxi Plasmid Kit Endotoxin Free
Cat. #PIF025PIFE25PI025PIE25PM010/25PME10/25
FormatAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity Flow
Binding Capacity350 µg350 µg350 µg350 µg1.0 mg1.0 mg
Filter ColumnYesYesN/AN/AN/AN/A
Endotoxin Level<0.15 EU/µg<0.10 EU/µg<0.15 EU/µg<0.10 EU/µg<0.15 EU/µg<0.10 EU/µg
Culture Input50-150 ml50-150 ml50-300 ml50-300 ml200-1600 ml200-1600 ml
Plasmid Size1-20 kb1-20 kb1-20 kb1-20 kb1-20 kb1-20 kb
Typical Yield350 µg350 µg350 µg350 µg1.0 mg1.0 mg
Elution Volume500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml
Operation Time<80 minutes<80 minutes<80 minutes<80 minutes<100 minutes<100 minutes

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