FroggaBio USA Inc - Geneaid Midi Plasmid Kit

The Geneaid™ Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells

TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization

• A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNARNA contaminants
• Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed
• The purified plasmid DNA is eluted then precipitated with isopropanol for desalting
• The entire procedure can be completed without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun
• **Available Endotoxin-Free, see product details below!** 

• Applications

  • Transfection
    
  • Sequencing
  • Ligation
  • PCR
  • In-vitro Transcription
  • Microinjection
  • Restriction Enzyme Digestion
  • Gene Gun
  • Components

  • • PM1, PM2, PM3 Buffer
    • TrueBlue Lysis Buffer
    • PEQ Buffer
    • PW Buffer
    • PEL Buffer
    • RNase A
    • Plasmid Midi Columns