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FroggaBio USA Inc - Midiprep Spin Column Plasmid Kit
Theandnbsp;High-Speed Plasmid Advance Kitandnbsp;was designed for plasmid and cosmid DNA purificationandnbsp;fromandnbsp;50-100 ml of cultured bacterial cellsandnbsp;using a plasmid midiprep spin column system
| Product description | Catalog # | Quantity | Price | |
|---|---|---|---|---|
|
PA002 |
|
$60.00 |
||
|
PA025 |
|
$570.00 |
FroggaBio USA Inc - Midiprep Spin Column Plasmid Kit Product Description
Theandnbsp;High-Speed Plasmid Advance Kitandnbsp;was designed for plasmid and cosmid DNA purificationandnbsp;fromandnbsp;50-100 ml of cultured bacterial cellsandnbsp;using a plasmid midiprep spin column system
The High-Speed Plasmid Advance Kit was designed for plasmid and cosmid DNA purification from 50-100 ml of cultured bacterial cells using a plasmid midiprep spin column system
This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNARNA contaminants
- In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
-
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water
-
- Typical yields are 200-350 μg for high-copy number plasmid or 30-100 μg for low-copy number plasmid from 50 ml of cultured bacterial cells
-
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 35 minutes
-
Applications
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PD1, PD2, PD3 Buffers
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PA Columns
-
This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNARNA contaminants
- In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
-
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water
-
- Typical yields are 200-350 μg for high-copy number plasmid or 30-100 μg for low-copy number plasmid from 50 ml of cultured bacterial cells
-
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 35 minutes
-
Applications
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PD1, PD2, PD3 Buffers
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PA Columns
-
- In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water
- Typical yields are 200-350 μg for high-copy number plasmid or 30-100 μg for low-copy number plasmid from 50 ml of cultured bacterial cells
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 35 minutes
-
- Restriction Enzyme Digestion
- Library Screening
- Ligation
- PCR
- Transformation and Sequencing Reactions
-
- PD1, PD2, PD3 Buffers
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
- RNase A
- PA Columns
-
Components
Applications
Accessories
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