My Cart: 0 item(s)
Edit Cart
QUICK ORDER
FroggaBio USA Inc - Presto 96 Well Plasmid Kit (Vacuum or Centrifuge)
Theandnbsp;Prestoandtrade; 96 Well Plasmid Kitandnbsp;was designed for high-throughput purification of plasmid DNA from 1-5 ml of cultured bacterial cells per well using an efficientandnbsp;96 well filter plateandnbsp;andandnbsp;96 well binding plate system
| Product description | Catalog # | Quantity | Price | |
|---|---|---|---|---|
|
96PDC02 |
|
$562.50 |
||
|
96PDV02 |
|
$605.00 |
||
|
96PDC04 |
|
$1,000.00 |
||
|
96PDV04 |
|
$1,100.00 |
||
|
96PDC10 |
|
$2,177.50 |
||
|
96PDV10 |
|
$2,480.00 |
FroggaBio USA Inc - Presto 96 Well Plasmid Kit (Vacuum or Centrifuge) Product Description
Theandnbsp;Prestoandtrade; 96 Well Plasmid Kitandnbsp;was designed for high-throughput purification of plasmid DNA from 1-5 ml of cultured bacterial cells per well using an efficientandnbsp;96 well filter plateandnbsp;andandnbsp;96 well binding plate system
The Presto™ 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well filter plate and 96 well binding plate system
A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
Typical yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes
-
- The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
Applications
- Fluorescent and Radioactive Sequencing
-
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- Transformation
-
- PCR
-
Components
- PD1, PD2, PD3 Buffers
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- Presto™ Plasmid 96 Well Filter Plates
-
- Presto™ Plasmid 96 Well Binding Plates
-
- 96 Deep Well Plates
-
- 035 ml Collection Plates
-
- Adhesive Film
-
A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
Typical yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes
-
- The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
Applications
- Fluorescent and Radioactive Sequencing
-
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- Transformation
-
- PCR
-
Components
- PD1, PD2, PD3 Buffers
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- Presto™ Plasmid 96 Well Filter Plates
-
- Presto™ Plasmid 96 Well Binding Plates
-
- 96 Deep Well Plates
-
- 035 ml Collection Plates
-
- Adhesive Film
-
Typical yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes
-
- The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
Applications
- Fluorescent and Radioactive Sequencing
-
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- Transformation
-
- PCR
-
Components
- PD1, PD2, PD3 Buffers
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- Presto™ Plasmid 96 Well Filter Plates
-
- Presto™ Plasmid 96 Well Binding Plates
-
- 96 Deep Well Plates
-
- 035 ml Collection Plates
-
- Adhesive Film
-
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes
- The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
- Fluorescent and Radioactive Sequencing
- Restriction Enzyme Digestion
- Library Screening
- Ligation
- Transformation
- PCR
-
- PD1, PD2, PD3 Buffers
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
- RNase A
- Presto™ Plasmid 96 Well Filter Plates
- Presto™ Plasmid 96 Well Binding Plates
- 96 Deep Well Plates
- 035 ml Collection Plates
- Adhesive Film
-
Components
Applications
Specifications
Format: 96 Well Plate
Binding Capacity: 35 µg
Culture Input: 1-5 ml
Plasmid Size: 1-15 kb
Typical Yield: 20-35 µgwell
Elution Volume: 100 µl
Operation Time: 25 minutes
**See product descriptions below to choose Vacuum or Centrifuge options**
Accessories
Product Promotions
Related Products
The new High Speed Microplate Shaker is up for Complex Jobs. The new OHAUS High Speed Microplate Shaker is one of our most versatile lab devices, capable of handling high-volume plate preparation as easily as it can the smallest adjustments.
See more
Improve Yields by Centrifuging plates before PCR to ensure all reactants are in the bottom of the wells for proper concentrations.
All the thrills... With NO SPILLS.
PATENTED Rotor Design allows plates to be inserted at an angle to avoid sample spillage. When the centrifuge is spinning, the plate holders automatically swing outward to hold the plates at a full 90° angle for flat, even accumulation of sample.
See more
EASYseal™ – adhesive sealer for everyday laboratory requirements: coverage/storage; protection against evaporation and contamination VIEWseal™ – highly transparent film for precise optical measurements: ideally suited for protein
crystallography and Real-Time PCR.
See more