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FroggaBio USA Inc - Presto Endotoxin Free Mini Plasmid Kit
The Prestoandtrade; Endotoxin Free Mini Plasmid Kit was designed for transfection grade plasmid DNApurification from 1-5 ml of cultured E coli cells using silica-membrane spin columnsandnbsp;
| Product description | Catalog # | Quantity | Price | |
|---|---|---|---|---|
|
PEH004 |
|
$10.04 $8.76 |
||
|
PEH100 |
|
$217.50 |
FroggaBio USA Inc - Presto Endotoxin Free Mini Plasmid Kit Product Description
The Prestoandtrade; Endotoxin Free Mini Plasmid Kit was designed for transfection grade plasmid DNApurification from 1-5 ml of cultured E coli cells using silica-membrane spin columnsandnbsp;
The Presto™ Endotoxin Free Mini Plasmid Kit was designed for transfection grade plasmid DNApurification from 1-5 ml of cultured E coli cells using silica-membrane spin columns
A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
In addition, an efficient endotoxin removal step is integrated in the procedure and endotoxins are precipitated in a proprietary reagent
TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes
-
- The purified endotoxin free plasmid DNA is ready for use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
Applications
- Transfection
-
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PE1, PE2, PE3, PE4, PE5 Buffers
-
- TrueBlue Lysis Buffer
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PDH Columns
-
- 2 ml Collection Tubes
-
A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
In addition, an efficient endotoxin removal step is integrated in the procedure and endotoxins are precipitated in a proprietary reagent
TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes
-
- The purified endotoxin free plasmid DNA is ready for use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
Applications
- Transfection
-
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PE1, PE2, PE3, PE4, PE5 Buffers
-
- TrueBlue Lysis Buffer
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PDH Columns
-
- 2 ml Collection Tubes
-
In addition, an efficient endotoxin removal step is integrated in the procedure and endotoxins are precipitated in a proprietary reagent
TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes
-
- The purified endotoxin free plasmid DNA is ready for use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
Applications
- Transfection
-
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PE1, PE2, PE3, PE4, PE5 Buffers
-
- TrueBlue Lysis Buffer
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PDH Columns
-
- 2 ml Collection Tubes
-
TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes
-
- The purified endotoxin free plasmid DNA is ready for use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
Applications
- Transfection
-
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PE1, PE2, PE3, PE4, PE5 Buffers
-
- TrueBlue Lysis Buffer
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PDH Columns
-
- 2 ml Collection Tubes
-
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes
- The purified endotoxin free plasmid DNA is ready for use in transfection, restriction enzyme digestion, ligation, PCR, and sequencing reactions
-
- Transfection
- Restriction Enzyme Digestion
- Library Screening
- Ligation
- PCR
- Transformation and Sequencing Reactions
-
- PE1, PE2, PE3, PE4, PE5 Buffers
- TrueBlue Lysis Buffer
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
- RNase A
- PDH Columns
- 2 ml Collection Tubes
-
Components
Applications
- Transfection
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