FroggaBio USA Inc - Presto Mini Plasmid Kit

This Plasmid Kit was designed for plasmid and cosmid DNA purification from 1-7 ml of cultured bacterial cells

For processing larger volumes, thePresto™ Midi Plasmid Kit is also available

The Presto™ Mini Plasmid Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNARNA contaminants

• Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors while ensuring efficient cell lysis, neutralization and maximum plasmid product yield In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
• Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water
• DNA phenol extraction or alcohol precipitation are not required and the entire procedure can be completed within 15 minutes 

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  Applications

  • Restriction Enzyme Digestion
  • Library Screening
  • Ligation
  • PCR
  • Transformation and Sequencing Reactions

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    Components

    • PD1, PD2, PD3 Buffers
    • TrueBlue Lysis Buffer
    • W1 Buffer
    • Wash Buffer
    • Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
    • RNase A
    • PDH Columns
    • 2 ml Collection Tubes