Mastercycler® X50a aluminum block, 96-well plate or 0.1/0.2 mL tubes, 100-240 V/50-60 Hz

PCR Upgraded: Savings on the Mastercycler® X50a with 2D gradient

The Eppendorf Mastercycler® X50a represents the next stage of PCR innovation, combining breakthrough 2D-Gradient optimization technology with exceptional speed and reliability for advanced molecular biology applications. This aluminum block thermal cycler accommodates 96-well plates or 0.1/0.2 mL tubes and features Eppendorf's revolutionary 2D-Gradient capability that allows simultaneous optimization of both annealing and denaturation temperatures across 12 columns and 8 rows in a single run. With heating rates up to 5 °C/s and cooling rates up to 2.3 °C/s, the X50a delivers significantly faster PCR protocols compared to conventional thermal cyclers, reducing total run times while maintaining superior temperature accuracy (±0.15 °C) and exceptional block homogeneity (≤±0.2 °C at 20–72 °C).

Engineered for both research flexibility and routine standardization, the Mastercycler X50a features an intuitive touchscreen interface with highly visual programming that puts advanced functionality at your fingertips. The instrument's excellent temperature control is achieved through six individually controlled Peltier elements that enable dynamic homogeneity regulation in real-time, ensuring consistent results from well to well and run to run. The innovative flexlid® concept provides automatic height adjustment to accommodate all types of consumables, while the gradient temperature range of 30–99 °C with adjustable gradient spans of 1–30 °C enables comprehensive optimization for challenging templates including GC-rich sequences and structures prone to forming hairpins.

The compact footprint with front-to-back ventilation saves valuable bench space without compromising on connectivity—up to 10 units can be networked without additional hardware, or up to 50 units via PC control for high-throughput laboratories. The remarkably quiet operation, advanced user management capabilities, and comprehensive documentation features make the X50a ideal for laboratories requiring validated workflows in food testing, diagnostics, and regulated environments. Complete with adjustable verification settings, transparent performance data export, and compatibility with Eppendorf's VisioNize Lab Suite for remote monitoring and alarm management, the Mastercycler X50a delivers professional-grade PCR performance with the standardization and documentation capabilities demanded by modern quality management systems.

Pipette.com is an official distributor of Eppendorf.