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Molecular Designs - Bacterial Medium:LB Agar - C121
LB Agar pre-formulated Luria-Bertani solid bacterial growth medium in powder form, 500g
Product Description:
LB Agar is a pre-formulated Luria-Bertani solid bacterial growth medium in powder form, designed for preparation of agar plates for bacterial plating, colony isolation, and transformation selection. At 35g per liter, a 500g package prepares approximately 14 liters of pourable medium -- enough for roughly 280 standard 50ml plates.
Technical Specifications:
- Format: Powder
- Package size: 500g
- Standard concentration: 35g/L
- pH: 7.0 ± 0.2 at 25°C
- Autoclave compatible
Composition per Liter:
- Tryptone: 10g
- Yeast Extract: 5g
- Sodium Chloride: 10g
- Agar: 10g
Preparation Protocol:
1. Weigh 35g powder per liter
2. Add appropriate volume of water
3. Mix until suspended
4. Autoclave at 121°C for 15 minutes
5. Cool to ~55°C before pouring plates
Research Applications:
- Bacterial plating
- Colony isolation
- Strain maintenance
- Transformation selection
- Microbiology research
- Cloning procedures
Storage Requirements:
- Store powder at room temp