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Eppendorf BioPhotometerD30 Spectrophotometer
The Eppendorf BioPhotometer D30 is the third generation of Eppendorf's well-established line of BioPhotometers. The small instrument clearly processes data, records at fixed wavelengths and makes evaluating results fast and simple. For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify.
| Product description | Catalog # | Quantity | Price | |
|---|---|---|---|---|
|
6133000010 |
|
$10,409.99 $8,756.67 |
Product Description:
The Eppendorf BioPhotometer D30 is the third generation of Eppendorf's well-established line of BioPhotometers. The small instrument clearly processes data, records at fixed wavelengths and makes evaluating results fast and simple. For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify.
Eppendorf BioPhotometer D30 - An Established Standard in the Field of Life Science
The Eppendorf BioPhotometer D30 is the 3rd generation of Eppendorf BioPhotometers, which have become an established standard in the field of life sciences. It utilizes fixed wavelengths, making the Eppendorf BioPhotometer D30 the ideal device for quick and precise routine applications. The ultra-stable Xenon light source quickly lights up for immediate instrument use. Data is clearly processed making the evaluation of results fast and simple while minimizing mistakes. Additional help menus assist the user and offer an additional level of security. Relevant ratios are automatically determined for the corresponding applications. The Eppendorf BioPhotometer D30 offers the option of recording a scan for specific applications in the UV range. This allows a quick visual identification of contaminants in the sample. This instrument is ideal for molecular analytics such as the assessment of nucleic acid purity and quantity.
Eppendorf BioPhotometer D30 features and benefits:
- Absorption measurements for one or more fixed wavelengths (range of 180nm to 2000nm).
- Scan for specific UV applications with automatically determined purity ratios.
- Preprogrammed applications for quick start and applications with evaluation via factor, standard or standard series.
- Integrated application and results memory.
- Data transfer via USB interface, Ethernet or via e-mail and a direct printout of results.
- Xenon flash lamp with very long lifetime.
- Guided software process to minimize errors.
- Help box with an explanation of each individual step in 5 languages.
- Compatible with standard cuvettes as well as microvolume measuring cells (allowing for the measurement of microvolumes of the sample as small as 1.5 μL - ideal for RNA and DNA measurements).
- Integrated self-test and calibration history.
View our full range of Cuvettes
Technical Specifications:
| Eppendorf BioPhotometer® D30 | |
| Absorption measuring range | 0?A?–?3.0?A (260?nm) |
| BSA concentration range | 7.5 ng/µL – 45.45 µg/µL |
| BSA concentration range (UV 280 nm) µCuvette G1.0 | 75 ng/µL – 45.45 µg/µL |
| ±0.03 µg/µL at A = 0 | |
| ±0.076 µg/µL at A = 1 (0.5 %) | |
| BSA concentration range (UV 280 nm) UVette 10 mm | 7.5 ng/µL – 4.5 µg/µL |
| ±0.003 µg/µL at A = 0 | |
| ±0.007 µg/µL at A = 1 (0.5 %) | |
| BSA concentration range (UV 280 nm) UVette 2 mm | 37.5 ng/µL – 22.5 µg/µL |
| ±0.015 µg/µL at A = 0 | |
| ±0.038 µg/µL at A = 1 (0.5 %) | |
| Beam receiver absorption | cm S photodiodes |
| Cuvette shaft | 12.5?mm?×?12.5?mm |
| Emission wavelength I fluorescence | NA |
| Emission wavelength II fluorescence | NA |
| Excitation wavelength fluorescence | NA |
| Fluorescence measuring range | NA |
| Light path height | 8.5 mm |
| Light source absorption | Xenon flash lamp |
| Light source fluorescence | NA |
| Measuring principle absorption | Single-beam absorption photometer with reference beam |
| Measuring principle fluorescence | NA |
| Method memory | >100 method programs |
| Operator guidance language | Spanish, Italian, French, English, German |
| Power consumption | Approx. 15?W during operating step |
| Approx. 5?W with dimmed display | |
| Random error absorption | ≤0.002 at A?=?0 |
| ≤0.005 (0.5?%) at A?=?1 | |
| Random error fluorescence | NA |
| Random error wavelength | ±1 nm |
| Random temperature error | NA |
| Spectral bandwidth | ≤4 nm |
| Systematic error absorption | ±1 % (A = 1) |
| Systematic error wavelength | ≤0.5 nm |
| Systematic temperature error | NA |
| Wavelength range absorption | Fixed wavelength (nm): 230, 260, 280, 320, 340, 405, 490, 562, 595, 600 |
| Wavelengths | 230, 260, 280, 320, 340, 405, 490, 562, 595, 600 nm |
| dsDNA concentration range | 2.5 ng/µL – 1,500 ng/µL |
| dsDNA concentration range (UV 260 nm) UVette 10 mm | 2.5 ng/µL – 150 ng/µL |
| ±0.1 ng/µL at A = 0 | |
| ±0.25 ng/µL at A = 1 (0.5 %) | |
| dsDNA concentration range (UV 260 nm) UVette 2 mm | 12.5 ng/µL – 750 ng/µL |
| ±0.5 ng/µL at A = 0 | |
| ±1.25 ng/µL at A = 1 (0.5 %) | |
| dsDNA concentration range (UV 260 nm) µCuvette G1.0 | 25 ng/µL – 1,500 ng/µL |
| ±1 ng/µL at A = 0 | |
| ±2.5 ng/µL at A = 1 (0.5 %) | |
| Interfaces | › USB master: for USB stick and thermal printer DPU-S445 |
| › USB slave for connecting to a PC | |
| › Serial interface RS-232: for thermal printer DPU-414 | |
| › Ethernet interface RJ45: For connecting to a network printer | |
| Power supply | 120 V, 50?–?60 Hz |
| Dimensions (W?×?D?×?H) | 295?× 400?× 150?mm?/ 11.6?× 15.7?× 6?in |
| Weight w/o accessories | 5.4 kg |
| Interval between two measuring points | NA |
| Measuring time range | NA |
| Adjustable temperature range | NA |
Eppendorf BioPhotometer Applications:
- Nucleic acid quantification.
- Direct protein quantification (UV 280 nm).
- Microvolume measurement via µCuvette G1.0 for highly concentrated samples.
- Bacteria growth measurement (OD 600).
- Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry.
- 340 nm: assays using NADPH or NAPH.
- 405 nm: assays using para-nitrophenol.
- 490 nm: cytotoxicity assay.
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