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Eppendorf BioPhotometerD30 Spectrophotometer

The Eppendorf BioPhotometer D30 is the third generation of Eppendorf's well-established line of BioPhotometers. The small instrument clearly processes data, records at fixed wavelengths and makes evaluating results fast and simple. For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify.

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Product description Catalog # Quantity Price

6133000010

$10,409.99

$8,756.67

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Eppendorf BioPhotometerD30 Spectrophotometer Product Description

The Eppendorf BioPhotometer D30 is the third generation of Eppendorf's well-established line of BioPhotometers. The small instrument clearly processes data, records at fixed wavelengths and makes evaluating results fast and simple. For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify.

BioPhotometer-D30-User-Manual

Technical Specifications:

Eppendorf BioPhotometer® D30
Absorption measuring range 0?A?–?3.0?A (260?nm)
BSA concentration range 7.5 ng/µL – 45.45 µg/µL
BSA concentration range (UV 280 nm) µCuvette G1.0 75 ng/µL – 45.45 µg/µL
±0.03 µg/µL at A = 0
±0.076 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 10 mm 7.5 ng/µL – 4.5 µg/µL
±0.003 µg/µL at A = 0
±0.007 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 2 mm 37.5 ng/µL – 22.5 µg/µL
±0.015 µg/µL at A = 0
±0.038 µg/µL at A = 1 (0.5 %)
Beam receiver absorption cm S photodiodes
Cuvette shaft 12.5?mm?×?12.5?mm
Emission wavelength I fluorescence NA
Emission wavelength II fluorescence NA
Excitation wavelength fluorescence NA
Fluorescence measuring range NA
Light path height 8.5 mm
Light source absorption Xenon flash lamp
Light source fluorescence NA
Measuring principle absorption Single-beam absorption photometer with reference beam
Measuring principle fluorescence NA
Method memory >100 method programs
Operator guidance language Spanish, Italian, French, English, German
Power consumption Approx. 15?W during operating step
Approx. 5?W with dimmed display
Random error absorption ≤0.002 at A?=?0
≤0.005 (0.5?%) at A?=?1
Random error fluorescence NA
Random error wavelength ±1 nm
Random temperature error NA
Spectral bandwidth ≤4 nm
Systematic error absorption ±1 % (A = 1)
Systematic error wavelength ≤0.5 nm
Systematic temperature error NA
Wavelength range absorption Fixed wavelength (nm): 230, 260, 280, 320, 340, 405, 490, 562, 595, 600
Wavelengths 230, 260, 280, 320, 340, 405, 490, 562, 595, 600 nm
dsDNA concentration range 2.5 ng/µL – 1,500 ng/µL
dsDNA concentration range (UV 260 nm) UVette 10 mm 2.5 ng/µL – 150 ng/µL
±0.1 ng/µL at A = 0
±0.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) UVette 2 mm 12.5 ng/µL – 750 ng/µL
±0.5 ng/µL at A = 0
±1.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) µCuvette G1.0 25 ng/µL – 1,500 ng/µL
±1 ng/µL at A = 0
±2.5 ng/µL at A = 1 (0.5 %)
Interfaces › USB master: for USB stick and thermal printer DPU-S445
› USB slave for connecting to a PC
› Serial interface RS-232: for thermal printer DPU-414
› Ethernet interface RJ45: For connecting to a network printer
Power supply 120 V, 50?–?60 Hz
Dimensions (W?×?D?×?H) 295?× 400?× 150?mm?/ 11.6?× 15.7?× 6?in
Weight w/o accessories 5.4 kg
Interval between two measuring points NA
Measuring time range NA
Adjustable temperature range NA

Eppendorf BioPhotometer Applications:

  • Nucleic acid quantification.
  • Direct protein quantification (UV 280 nm).
  • Microvolume measurement via µCuvette G1.0 for highly concentrated samples.
  • Bacteria growth measurement (OD 600).
  • Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry.
  • 340 nm: assays using NADPH or NAPH.
  • 405 nm: assays using para-nitrophenol.
  • 490 nm: cytotoxicity assay.
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