FroggaBio USA Inc - Presto Buccal Swab gDNA Extraction Kit

The Presto™ Buccal Swab gDNA Extraction Kitprovides an efficient method for purifying DNA (including genomic, mitochondrial and viral DNA) from buccal cells

This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column 

• Carrier RNA is included with the kit to improve the efficiency of DNA binding to the spin column membrane
• Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol)
• The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water
• High quality genomic DNA can be purified in 20 minutes without phenolchloroform extraction or alcohol precipitation, with an average DNA yield of 2 μg per buccal swab
• Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions

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  Applications

  • PCR
  • Restriction Endonuclease Digestions
  • Array
  • Southern Blots and various other enzymatic reactions

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    Components

    • S1 Buffer
    • S2 Buffer
    • Carrier RNA
    • W1 Buffer
    • Wash Buffer
    • Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
    • Proteinase K
    • GD Columns
    • Filter Columns
    • 2 ml Collection Tubes