PCR

Laboratory PCR workflows demand precision, contamination-free conditions and reliable consumables to ensure accurate DNA amplification and quantification. 

Essential tools include manual and electronic pipettes, particularly positive-displacement or multichannel pipettes (e.g. 384channel systems) optimized for PCR/qPCR work, paired with PCR clean tips and pipettes that minimize aerosol contamination. PCR Reaction Vessels form the foundation of successful amplification, including singletube and 8strip PCR tubes, specialized PCR plates – 96well and 384well PCR plates (including white plates designed to enhance fluorescence detection in qPCR), all certified free of DNA/DNase/RNase and PCR inhibitors. 

Thermal Management Systems provide precise temperature control essential for PCR success. Thermal cyclers provide controlled temperature cycling through denaturation, annealing, and extension phases necessary for amplification of target sequences. 

Supporting equipment like vortex mixers assist with thorough sample homogenization, centrifuges ensure sample clarification, heat sealers (or plate seal films) help prevent evaporation and contamination during thermal cycling, while pipette stands and racks ensure efficient setup and handling of reaction plates and tubes. 

Pipette.com offers the complete PCR ecosystem with specialized pipettes, tubes, plates and accessories tailored to PCR and qPCR workflows, backed by ISO17025 calibration services to ensure accuracy and contamination control.