Pipette.com Agarose - Low Melt

The ideal agarose for low melting and gelling applications.

Low Melting (LM) agaroses are derivatized by organic synthesis which generates methoxylate groups from the basic agarose structure.

The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature

The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where In-Gel manipulations can be performed without prior extraction of the DNA from the gel slice.

Features

• Lower gel strength than the standard agaroses. Even so, gels can be handled easily.
• Higher clarity (gel transparency) than gels of standard agaroses.
• Greater sieving capacity

LM agaroses are classified in three categories, depending on the degree of derivatization. Gelling/melting temperatures and gel strength are the most important differences.

Applications

LM (Low Melt): with the highest gelling/melting temperatures and gel strength.

• Electrophoresis of DNA fragments ≥1000 bp
• Tissue and cell culture
• Viral plaque assays

S.LM (Super Low Melt): with lower gelling/melting temperatures and lower gel strength than LM

• Capillary electrophoresis
• Tissue and cell culture
• Viral plaque assays

E.LM (Extra Low Melt): with the lower gelling/melting temperatures and lower gel strength than S.LM

• Capillary electrophoresis
• Tissue and cell culture
• Viral plaque assays

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