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Enrichment of disseminated, circulating tumor cells from peripheral blood
- Time request: approximately 45 minutes
- Reproducible recovery: > 70 %
- Depletion of blood cells by up to 6 log units
- No additional laboratory equipment requiredNo magnetic beads needed
- No blocking of marker molecules
Enrichment directly from whole blood
OncoQuick® is a simple-to-use, rapid and efficient system for the enrichment of circulating tumor cells that are released into the blood by a solid epithelial tumor or malignant melanoma.
OncoQuick® combines the advantages of cell separation by density gradient centrifugation (rapid, reproducible and cost- effective) with recovery rates that are comparable with immunobead methods.
OncoQuick® consists of a sterile 50 mL polypropylene tube with a porous barrier which is inserted above the specially developed separation medium.
Up to 30 mL of anticoagulated whole blood is directly filled into the OncoQuick® tube and centrifuged. Apart from erythrocytes and granulocytes, the separation medium also allows the elimination of lymphocytes and mononuclear cells to a wide extent. The disseminated tumor cells are enriched in the interphase. After harvesting, the enriched cell fraction is washed. The tumor cells are then available for all standard research methods.