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FroggaBio USA Inc - Large Plasmid DNA Extraction Kit

Theandnbsp;High-Speed Plasmid Mini Kit (10-50 Kb)andnbsp;was designed for plasmid and cosmid DNA purification from 1-4 ml of cultured bacterial cells

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PDL004

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FroggaBio USA Inc - Large Plasmid DNA Extraction Kit Product Description

Theandnbsp;High-Speed Plasmid Mini Kit (10-50 Kb)andnbsp;was designed for plasmid and cosmid DNA purification from 1-4 ml of cultured bacterial cells

The High-Speed Plasmid Mini Kit (10-50 Kb) was designed for plasmid and cosmid DNA purification from 1-4 ml of cultured bacterial cells

This plasmid kit is optimized for isolating 10-50 kb plasmid DNA using a miniprep spin column system 

  • High-Speed Plasmid Mini Kit (10-50 Kb) uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
  • In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
  • Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE
  • Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
  • DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes
  • The purified plasmid DNA is ready for use in Restriction Enzyme Digestion, Ligation, PCR, and sequencing reactions
    •  

      Applications

      • Restriction Enzyme Digestion
      • Library Screening
      • Ligation
      • PCR
      • Transformation and Sequencing Reactions
        •  

          Components

          • PDL1, PDL2, PDL3 Buffers
          • WL1 Buffer
          • Wash Buffer
          • Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
          • RNase A
          • LP Columns
          • 2 ml Collection Tubes

Specifications

 

Format: Spin Column

Binding Capacity: 30 µg

Culture Input: 1-4 ml

Plasmid Size: 10-50 kb

Typical Yield: 10-25 µg

Elution Volume: 50-100 µl

Operation Time: <15 minute

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