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FroggaBio USA Inc - Large Plasmid DNA Extraction Kit
Theandnbsp;High-Speed Plasmid Mini Kit (10-50 Kb)andnbsp;was designed for plasmid and cosmid DNA purification from 1-4 ml of cultured bacterial cells
| Product description | Catalog # | Quantity | Price | |
|---|---|---|---|---|
|
PDL004 |
|
$10.04 $8.76 |
||
|
PDL100 |
|
$217.50 |
||
|
PDL300 |
|
$587.50 |
FroggaBio USA Inc - Large Plasmid DNA Extraction Kit Product Description
Theandnbsp;High-Speed Plasmid Mini Kit (10-50 Kb)andnbsp;was designed for plasmid and cosmid DNA purification from 1-4 ml of cultured bacterial cells
The High-Speed Plasmid Mini Kit (10-50 Kb) was designed for plasmid and cosmid DNA purification from 1-4 ml of cultured bacterial cells
This plasmid kit is optimized for isolating 10-50 kb plasmid DNA using a miniprep spin column system
- High-Speed Plasmid Mini Kit (10-50 Kb) uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
-
- In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
-
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE
-
- Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
-
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes
-
- The purified plasmid DNA is ready for use in Restriction Enzyme Digestion, Ligation, PCR, and sequencing reactions
-
Applications
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PDL1, PDL2, PDL3 Buffers
-
- WL1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- LP Columns
-
- 2 ml Collection Tubes
-
This plasmid kit is optimized for isolating 10-50 kb plasmid DNA using a miniprep spin column system
- High-Speed Plasmid Mini Kit (10-50 Kb) uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
-
- In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
-
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE
-
- Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
-
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes
-
- The purified plasmid DNA is ready for use in Restriction Enzyme Digestion, Ligation, PCR, and sequencing reactions
-
Applications
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PDL1, PDL2, PDL3 Buffers
-
- WL1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- LP Columns
-
- 2 ml Collection Tubes
-
- High-Speed Plasmid Mini Kit (10-50 Kb) uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants
- In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE
- Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
- DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes
- The purified plasmid DNA is ready for use in Restriction Enzyme Digestion, Ligation, PCR, and sequencing reactions
-
- Restriction Enzyme Digestion
- Library Screening
- Ligation
- PCR
- Transformation and Sequencing Reactions
-
- PDL1, PDL2, PDL3 Buffers
- WL1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
- RNase A
- LP Columns
- 2 ml Collection Tubes
-
Components
Applications
Specifications
Format: Spin Column
Binding Capacity: 30 µg
Culture Input: 1-4 ml
Plasmid Size: 10-50 kb
Typical Yield: 10-25 µg
Elution Volume: 50-100 µl
Operation Time: <15 minute
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