FroggaBio USA Inc - Presto DNA/RNA Extraction Kit

The Presto™ DNA RNA Extraction Kit provides an efficient method for purifying genomic DNA and total RNA simultaneously from cultured cells, animal tissue, whole blood and biological fluids

Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the genomic DNA mini spin column

• The flow-through containingRNA can then be transferred to the RNA mini spin column for RNA binding
• Contaminants are removed using a wash buffer and the purified genomic DNA is eluted in a low salt Elution Buffer and the purified total RNA is eluted in RNase-free Water
• The entire procedure can be completed in 25 minutes without phenolchloroform extraction or alcohol precipitation
• The purified DNA, approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis

• Applications

  The purified DNA, approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis

  Components

  • RBC Lysis Buffer
  • DR Buffer
  • RW1 Buffer
  • RPE Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • Elution Buffer (10 mM Tris-HCl, pH85 at 25ºC)
  • GD Columns
  • RB Columns
  • 2 ml Collection Tubes

  • Quality Control

    The quality of the Presto™ DNA RNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA and total RNA from 1 x 10^{6 cultured animal cells The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel}