FroggaBio USA Inc - gSYNC DNA Extraction Kit

TissueBlood DNA Mini Kit (GS100, GS300)

The gSYNC™ DNA Extraction Kit is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood (fresh blood and frozen blood), serum, plasma, buffy coat, body fluids, cultured cells, tissue, rodent tails, ear punches, formalin-fixed paraffin-embedded tissue (FFPE), amniotic fluid, hair, insects and sperm in one convenient kit 

This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column

Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water

The entire procedure can be completed in less than 20 minutes without phenolchloroform extraction or alcohol precipitation Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions

Applications

• PCR,
• AFLPPADP,
• RFLP,
• Southern Blotting,
• Real-time PCR

• Components

  • GST Buffer
    • 30 ml
    • • GSB Buffer
        • 40 ml
        • • W1 Buffer
            • 45 ml
            • • Wash Buffer
                • 25 ml (add ethanol 100ml)
                • • Elution Buffer (10 mM Tris-HCl, pH85 at 25ºC) 
                    • 30 ml
                    • • Proteinase K
                        • 11mg X 2 (110 ml)
                        • • GS Columns
                            • 100
                            • • 2 ml Collection Tubes
                                • 200

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