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FroggaBio USA Inc - High-Speed Plasmid Mini Kit
This plasmid kitandnbsp;will purify plasmid DNAcosmid DNA from 1-5 ml of cultured bacterial cells using an efficient plasmidandnbsp;miniprepandnbsp;systemandnbsp;
| Product description | Catalog # | Quantity | Price | |
|---|---|---|---|---|
|
PD004 |
|
$10.04 $8.76 |
||
|
PD100 |
|
$176.80 |
||
|
PD300 |
|
$452.50 |
FroggaBio USA Inc - High-Speed Plasmid Mini Kit Product Description
This plasmid kitandnbsp;will purify plasmid DNAcosmid DNA from 1-5 ml of cultured bacterial cells using an efficient plasmidandnbsp;miniprepandnbsp;systemandnbsp;
This plasmid kit will purify plasmid DNAcosmid DNA from 1-5 ml of cultured bacterial cells using an efficient plasmid miniprep system
The High-Speed Plasmid Mini Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNARNA contaminants In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water
-
- Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
-
- This plasmid kit does not require DNA phenol extraction or alcohol precipitation and the entire procedure can be completed within 15 minutes
-
Applications
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PD1, PD2, PD3 Buffers
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PD Columns
-
- 2 ml Collection Tubes
-
The High-Speed Plasmid Mini Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNARNA contaminants In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water
-
- Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
-
- This plasmid kit does not require DNA phenol extraction or alcohol precipitation and the entire procedure can be completed within 15 minutes
-
Applications
- Restriction Enzyme Digestion
-
- Library Screening
-
- Ligation
-
- PCR
-
- Transformation and Sequencing Reactions
-
Components
- PD1, PD2, PD3 Buffers
-
- W1 Buffer
-
- Wash Buffer
-
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
-
- RNase A
-
- PD Columns
-
- 2 ml Collection Tubes
-
- Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water
- Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells
- This plasmid kit does not require DNA phenol extraction or alcohol precipitation and the entire procedure can be completed within 15 minutes
-
- Restriction Enzyme Digestion
- Library Screening
- Ligation
- PCR
- Transformation and Sequencing Reactions
-
- PD1, PD2, PD3 Buffers
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
- RNase A
- PD Columns
- 2 ml Collection Tubes
-
Components
Applications
Specifications
Format: Spin Column
Binding Capacity: 30 µg
Culture Input: 1-5 ml
Plasmid Size: 1-15 kb
Typical Yield: 10-30 µg
Elution Volume: 30-100 µl
Operation Time: <15 minutes
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