FroggaBio USA Inc - Presto gDNA Bacteria Advanced Kit

The Presto™ gDNA Bacteria Advanced Kit is designed for rapid isolation of genomic DNA from cultured Gram (+) positive and Gram (-) negative bacteria

After directly adding into pre-filled beadbeating tubes containing zirconiaceramic beads and lysis buffer, bacterial cells are efficiently lysed using a standard vortex or bead beating instrument

• Enzymatic digestion with Lysozyme and Proteinase K is not required
• The cell lysate is then mixed with a binding buffer, followed by bind, wash and elute using a spin column designed specifically for bacteria DNA extraction
• DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 30 minutes
• The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions 

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  Applications

  • PCR
  • Restriction Endonuclease Digestions
  • Array
  • Southern Blots and various other enzymatic reactions

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    Components

    • GT Buffer
    • PR Buffer
    • GB Buffer
    • W1 Buffer
    • Wash Buffer
    • Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
    • RNase A (50 mgml)
    • Beadbeating Tubes Type A
    • GD Columns
    • 2 ml Collection Tubes

    • Presto™ Mini gDNA Bacteria Kit Functional Test Data Gram (-) Negative Bacteria

      Figure 1 Genomic DNA was extracted from 50 mg of Escherichia coli pellet? using the Presto™ gDNA Bacteria Advanced Kit A 2 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel M = Geneaid 1 Kb DNA Ladder Sample ngµl 260280 260230 Yield Test 1 3121 189 234 312 µg Test 2 3416 188 235 342 µg Test 3 3328 188 232 333 µg Presto™ Mini gDNA Bacteria Kit Functional Test Data Gram (+) Positive Bacteria  Figure 2 Genomic DNA was extracted rom 50 mg of Bacillus subtilis pellet using the Presto™ gDNA Bacteria Advanced Kit A 2 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel  M = Geneaid 1 Kb DNA Ladder Sample ngµl 260280 260230 Yield Test 1 1218 191 239 122 µg Test 2 1309 191 240 131 µg Test 3 1336 189 239 134 µg Specifications   Specifications gSYNC™ DNA Extraction Kit Genomic DNA Mini Kit Blood Cultured Cell Genomic DNA Mini Kit Tissue Genomic DNA MiniMaxi Kit Plant Presto™ Mini gDNA Bacteria Kit Presto™ Mini gDNA Yeast Kit Geneius™ Micro gDNA Extraction Kit Presto™ Buccal Swab gDNA Extraction Kit Cat # GS100 GS300 GB100 GB300 GT050 GT100 GT300 GP100 GPM010 GPM025 GBB100101 GBB300301 GBYB100 GBYB300 GMB100 GMB300 GSK100 GSK300 Format Spin Column Spin Column Spin Column Spin Column Spin Column Spin Column Spin Column Spin Column Binding Capacity 50 µg 50 µg 50 µg 50 µg (Mini) 500 µg (Maxi) 50 µg NA Accessories Product Promotions Related Products OHAUS High-Speed Microplate Shaker The new High Speed Microplate Shaker is up for Complex Jobs. The new OHAUS High Speed Microplate Shaker is one of our most versatile lab devices, capable of handling high-volume plate preparation as easily as it can the smallest adjustments. See more Shop Now Benchmark PlateFuge Microcentrifuge Improve Yields by Centrifuging plates before PCR to ensure all reactants are in the bottom of the wells for proper concentrations. All the thrills... With NO SPILLS. PATENTED Rotor Design allows plates to be inserted at an angle to avoid sample spillage. When the centrifuge is spinning, the plate holders automatically swing outward to hold the plates at a full 90° angle for flat, even accumulation of sample. See more Shop Now Greiner Plate Sealers EASYseal™ – adhesive sealer for everyday laboratory requirements: coverage/storage; protection against evaporation and contamination VIEWseal™ – highly transparent film for precise optical measurements: ideally suited for protein crystallography and Real-Time PCR. See more Shop Now Log In to Your Account Global Account Log In Email Address: Password: Forgot Password? Sign In Not a member? Create an account Guest Checkout Finalize your order without creating an account and you will have an option to create an account after placing the order. Continue As a Guest Reset Password In order to reset your password, please enter your email address: Forgot Password Email: Return to Log In Log In to Your Account Global Account Log In Email Address: Password: Forgot Password? Sign In Not a member? Create an account Reset Password In order to reset your password, please enter your email address: Forgot Password Email: Return to Log In