FroggaBio USA Inc - Presto Midi Plasmid Kit

The Presto™ Midi Plasmid Kit was designed for Plasmid DNA Purification from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep system 

For processing smaller volumes, the Presto™ Mini Plasmid Kit is also available

Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors, while ensuring efficient cell lysis and neutralization

• The Presto™ Midi Plasmid Kit uses a modified alkaline lysis method and RNase treatment  to obtain clear cell lysate with minimal genomic DNARNA contaminants
• Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound by the plasmid midi column and the contaminants are removed with PMC Buffer
• The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting
• The entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC or other toxic reagents  

• Applications

  • Transfection
  • Sequencing
  • Ligation
  • PCR
  • In-vitro Transcription
  • Microinjection
  • Restriction Enzyme Digestion
  • Gene Gun 
  • **Available Endotoxin-Free, see product descriptions**

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