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FroggaBio USA Inc - Presto Midi Plasmid Kit

Theandnbsp;Prestoandtrade; Midi Plasmid Kitandnbsp;was designed for Plasmid DNA Purificationandnbsp;from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep systemandnbsp;

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Product description Catalog # Quantity Price

PIF002

$55.00

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PIFE02

$75.00

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PIF025

$275.00

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PIFE25

$665.00

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FroggaBio USA Inc - Presto Midi Plasmid Kit Product Description

Theandnbsp;Prestoandtrade; Midi Plasmid Kitandnbsp;was designed for Plasmid DNA Purificationandnbsp;from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep systemandnbsp;

The Presto™ Midi Plasmid Kit was designed for Plasmid DNA Purification from 50-150 ml of cultured bacterial cells using an efficient plasmid midiprep system 

For processing smaller volumes, the Presto™ Mini Plasmid Kit is also available

Both plasmid kits include TrueBlue Lysis Buffer (an optional color indicator) to prevent common handling errors, while ensuring efficient cell lysis and neutralization

  • The Presto™ Midi Plasmid Kit uses a modified alkaline lysis method and RNase treatment  to obtain clear cell lysate with minimal genomic DNARNA contaminants
  • Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound by the plasmid midi column and the contaminants are removed with PMC Buffer
  • The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting
  • The entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC or other toxic reagents  
    • Applications

      • Transfection
      • Sequencing
      • Ligation
      • PCR
      • In-vitro Transcription
      • Microinjection
      • Restriction Enzyme Digestion
      • Gene Gun 
      • **Available Endotoxin-Free, see product descriptions**

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