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FroggaBio USA Inc - Presto Mini gDNA Bacteria Kit (with or without Lysozyme)

Theandnbsp;Prestoandtrade; Mini gDNA Bacteria Kitandnbsp;is a genomicandnbsp;DNAandnbsp;extractionandnbsp;kitandnbsp;optimized for genomic and viral DNA purification from Gramandnbsp;negative bacterial cells and Gram positive bacterial cells

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GBB004

$30.22785

$29.85

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GBB101

$445.00

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GBB100

$540.00

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GBB301

$1,200.00

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GBB300

$1,457.50

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FroggaBio USA Inc - Presto Mini gDNA Bacteria Kit (with or without Lysozyme) Product Description

Theandnbsp;Prestoandtrade; Mini gDNA Bacteria Kitandnbsp;is a genomicandnbsp;DNAandnbsp;extractionandnbsp;kitandnbsp;optimized for genomic and viral DNA purification from Gramandnbsp;negative bacterial cells and Gram positive bacterial cells

The Presto™ Mini gDNA Bacteria Kit is a genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells

ThisDNA Extraction Kit includes Gram+ Buffer, which combined with lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer

  • Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column
  • Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water 
  • The entire procedure can be completed within 20 minutes without phenolchloroform extraction or alcohol precipitation
  • The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions 
    •  

      Applications

      • PCR
      • Restriction Endonuclease Digestions
      • Array
      • Southern Blots and various other enzymatic reactions
        • Components

          • Gram+ Buffer
          • GT Buffer
          • GB Buffer
          • W1 Buffer
          • Wash Buffer
          • Elution Buffer (10 mM Tris-HCl, pH85 at 25°C)
          • GD Columns
          • 2 ml Collection Tubes

Specifications

Format: Spin Column

Binding Capacity: 50 µg (Mini) 500 µg (Maxi)

Sample: 1x109bacterial cells,200 µl blood,1 ml urine, plasma

Micropestle: NA

Filter Column: NA

Enzyme: Lysozyme, Proteinase K

Typical Yield: 37 µg from 1x109E coli, 10-15 µg from 1x109Bacillus subtilis

Elution Volume: 30-200 µl (Mini), 1 ml (Maxi)

Operation Time: <30 minutes

 

GBB100- w Lysozyme 100 prepskit  
GBB101- 100 prepskit
GBB300- w Lysozyme 300 prepskit
GBB301- 300 prepskit
GBB004- 4 prepskit

 

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