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FroggaBio USA Inc - UltraScience TMB Substrate

TMB Substrate, known as 3,3andrsquo;,5,5andrsquo;-tetramethylbenzidine, andnbsp;is a chromagen that detects horseradish peroxidase (HRP) activity when oxidized with hydrogen peroxide and yields a blue color with major absorbances at 370 and 652nm

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Product description Catalog # Quantity Price

TM001-B100ML

$60.75949

$60.00

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FroggaBio USA Inc - UltraScience TMB Substrate Product Description

TMB Substrate, known as 3,3andrsquo;,5,5andrsquo;-tetramethylbenzidine, andnbsp;is a chromagen that detects horseradish peroxidase (HRP) activity when oxidized with hydrogen peroxide and yields a blue color with major absorbances at 370 and 652nm

TMB Substrate, known as 3,3’,5,5’-tetramethylbenzidine, is a chromagen that detects horseradish peroxidase (HRP) activity when oxidized with hydrogen peroxide and yields a blue color with major absorbances at 370 and 652nm

The substrate is supplied as a ready to use, mildly acidic solution

  • The unreacted substrate should be colorless or very light yellow in appearance
  • The reaction can be stopped using appropriate stop solution, either the sulfuric or phosphoric acid, and producing a soluble yellow reaction product with the absorbance at 450nm
    • Unlike other commercially available substrates, Bio-Helix’s TMB contains no DMF

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