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Hamilton Company Ion Exclusion Columns
Ion exclusion chromatography is an alternative to ion exchange chromatography.
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Hamilton Company Ion Exclusion Columns Product Description
Ion exclusion chromatography is an alternative to ion exchange chromatography.
Ion exclusion chromatography is an alternative to ion exchange chromatography in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and non-ionic compounds elute later. Mixtures of weak acids, like those in fruits and milk products, are frequently not very well separated by pure ion-exchange methods, nor in the reversed-phase mode.
Packing Information
PRP-X300
Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrenedivinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes: hydrogen bonding, reversed-phase, and ion exclusion. PRP-X300 column selectivity can be altered by changing the pH of the buffer or adding an organic modifier (e.g., methanol, acetonitrile). The support’s stability to organic solvents makes it possible to analyze samples that are too highly retained on conventional ion exclusion supports.
Application Examples: Organic acids and alcohols
| Part Number | Hardware Inner Diameter | Hardware Length | Particle Size | Pore Size |
| 79915 | 4.6 mm | 33 mm | 12-20 µm | 0 |
| 79289 | 2.1 mm | 33 mm | 12-20 µm | 0 |
| 79373 | 3.0 mm | 8 mm | 12-20 µm | 0 |
| 79374 | 3.0 mm | 8 mm | 12-20 µm | 0 |
| 79453 | 2.1 mm | 20 mm | 12-20 µm | 0 |
| 79460 | 2.1 mm | 20 mm | 12-20 µm | 0 |
| 79465 | 4.1 mm | 250 mm | 7 µm | 100 Å |
| 79464 | 4.1 mm | 150 mm | 7 µm | 100 Å |
| 79475 | 4.6 mm | 150 mm | 7 µm | 100 Å |
| 79130 | 4.6 mm | 20 mm | 12-20 µm | 0 |
| 79129 | 4.6 mm | 20 mm | 12-20 µm | 0 |
| 79397 | 2.1 mm | 250 mm | 7 µm | 100 Å |