![Lamda Bio HotStart PCR 2x Master Mix in Green](graphics/00000001/2/D136G-2-228x228.jpg)
Lamda Bio HotStart PCR 2x Master Mix in Green
High performance, ready-to-use 2X PCR master mix with HotStart Taq DNA polymerase, in green color.
Product description | Catalog # | Quantity | Price | |
---|---|---|---|---|
G208-100 |
|
$200.34 $166.95 |
||
D136G |
|
$236.25 $182.57 |
||
MX-S |
|
$299.88 $249.90 |
||
MX-PS |
|
$332.64 $277.20 |
Lamda Bio HotStart PCR 2x Master Mix in Green Product Description
High performance, ready-to-use 2X PCR master mix with HotStart Taq DNA polymerase, in green color.
High performance, ready-to-use 2X PCR master mix with HotStart Taq DNA polymerase, in green color.
![](https://f.hubspotusercontent10.net/hubfs/2753166/Lambda%20Bio%20Protocols/D136G-2-228x228.jpg)
Users only need to add template, primers and H2O for the reaction. The HotStart PCR 2x Master Mix contains inert, non-toxic dyes to visualize PCR mixing step, and also allows direct loading of PCR products on to gels for electrophoresis.
- The HotStart Taq DNA Polymerase is a chemically modified Taq DNA Polymerase, whose enzyme activities can only be activated after 3-5 minutes of incubation at 95°C.
- The HotStart Taq Polymerase uses amplification conditions for regular Taq DNA Polymerase, except no polymerase activity will be present before the onset of thermal cycling.
- This prevents nonspecific DNA amplification and primer dimer formation.
- The amplified products, up to 7Kb in length, contain PCR products with blunt and 3’-overhanging A end.
- This allows flexible protocols for PCR cloning, if the amplified fragments need to be cloned.
SPECIAL FEATURES
- All-in-one 2X master mix format
- Hot start
- Pre-optimized
- Direct gel loading
- In green color
- up to 7kb in length
APPLICATIONS
- High Sensitivity PCR
- High Throughput PCR
- Routine PCR
- Colony PCR
- Hot Start PCR
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